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Ten synthetic iron chelators of catecholate type stimulated growth of a pyoverdine/pyochelin deficient P. aeruginosa PAO1 mutant under condition of severe iron limitation.
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Ectopic expression of the PduA protein in a Salmonella enterica pduA deletion mutant under conditions in which formation of the propanediol utilization (Pdu) microcompartment was induced resulted in a mixture of normal and abnormal microcompartments [25].
Thus, we examined the tSEC6 mutant under conditions that are optimal for hyphal growth on solid and in liquid media for up to 24 h.
It might be interesting to evaluate growth of the osrecql4 mutant under conditions of elevated UV, since DNA replication arrest is induced by UV photoproducts.
The level of SodB transcript appeared to be increased in the wild-type strain compared to the Δ perR mutant under conditions of iron restriction (with or without H2O2).
In summary, our microarray analysis of the transcription profile of a pppA-ppkA mutant under conditions of oxidative stress revealed that the response of the mutant strain to the oxidative stress is less coordinated than that of the wild-type strain.
To obtain quantitative data on the role of CIII in the absence of FtsH or HflK we carried out infections of bacterial mutant strains with λ wt and mutants under conditions that greatly favor the lysogenic pathway.
Mycobacterial resistance to anti-tuberculosis drugs initially emerges within treated hosts by the selection of rare, sporadically-occurring mutants under conditions of inadequate chemotherapy (acquired drug resistance; Fig. 2, arrow b).
Our investigations showed no growth defects in YuaG deletion mutants under conditions of cellular stress [M. Hinderhofer, unpublished data].
Alternatively, as SPB components expectedly remain insoluble upon protein extractions, we might not be able to show impaired recruitment to the SPB in the mutants under conditions currently used.
To circumvent this issue as much as possible, investigators have tried to evolve (in case of experimental evolution) or select (in case of the other approaches) strains or mutants under conditions very similar to the conditions in which the improved strain will be used in industry.
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