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In contrast, mutant type sev promoter activity was not changed by dNF-YAdsRNA as compared to LacZdsRNA treatment.
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Fragments originating from mutant type (M) and wild-type (W) OsIRE1 are indicated by closed arrowheads.
In parallel, multiplication of wild type SeV was evaluated by Western blot of the allantoic fluid with a monoclonal antibody (Mab) specific for the SeV F protein.
The authors went onto show that pDCs, in a MyD88-dependent manner, were the primary IFN producing cells in response to wild type SeV.
Transient luciferase expression assays were conducted with the wild type sev gene promoter-luciferase reporter gene, after treating S2 cells with dNF-YAdsRNA (dsdNF-YA) or LacZdsRNA (dsLacZ).
Treatment of cells with dNF-YAdsRNA reduced the wild type sev gene promoter activity by 27.4% as compared to control LacZdsRNA treatment (Fig. 6A).
The transgenic fly line carrying the wild type sev promoter and enhancer showed high expression of lacZ in photoreceptor cells appearing in the region posterior to the morphogenetic furrow (MF), as reported previously (Fig. 5D) (Basler et al., 1989).
Although wild-type SeV is known for its function of causing cell fusions, the SeV vectors used here lack the capacity for cell fusion.
And some studies suggest that the most enthusiastic anti-mutant types may be super-powered mutants themselves, albeit unknowingly.
When infected with mutant SeV strains, Traf6−/− MEF cells showed no detectable induction of IL-6 production compared with the substantial induction in Traf6+/+ MEF cells (Figure 1A, top).
We used the Cm and V-mutants of SeV; these mutations inhibit antiviral responses in host cells [37], [38].
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