The pCambia 1300 derivative was introduced into the GV3121 strain of Agrobacterium tumefaciens and the pbrp2-1 mutant plants transformed by the floral dipping method.
The A. thaliana plants (Col wild type or fld mutant) were transformed by the floral dip method [ 60].
Individual phosphinothricin (ppt) resistant heterozygous tio-3 mutants were transformed by floral dipping [ 45].
unc-119 mutant worms were transformed by biolistic transformation with a vector containing the wild-type unc-119 gene, the promoter of the constitutive and ubiquitously expressed rpl-17 gene, and the hyper coding region.
After transfer of the constructs into Agrobacterium tumefaciens (GV3101), Arabidopsis pbl1 mutant plants were transformed by floral-dip transformation.
Although enforced expression of PIK3CA could not induce OSE anchorage-independent growth, it significantly increased anchorage-independent growth of OSE transformed by mutant K-ras.
EPEC espD mutant strain UMD870 was transformed by electroporation.
slac1-1 mutant plants were then transformed by the floral dipping method (Clough and Bent, 1998) and propagated until the T-DNA insertion was confirmed to be homozygous.
Transcript expression analyses have been performed comparing normal rat embryo fibroblasts and cells transformed by mutant Hras, Kras, and Nras (Zuber et al, 2000).
The ability to enter a resting state is temperature-sensitive (ts) in cells transformed by a tsA mutant of simian virus 40.
