Sentence examples for mutant transcript is from inspiring English sources

The mutant transcript is a potential target for degradation by the nonsense mediated decay (NMD) pathway [11].

Although no one mutant transcript is common to all MSI-High tumours, a vaccine that targets a set of commonly mutated proteins may be an effective treatment for MSI-High colon cancers.

We suggest, therefore, that the wild type-like transcript is expressed mainly or exclusively in cone photoreceptors, accounting for the relative sparing of cone transmission as seen in the optokinetic response, and that the mutant transcript is expressed mainly or exclusively in rod photoreceptors, accounting for the striking loss of synaptic terminals and consequent thinning in the OPL.

RT-PCR analysis confirmed that the mutant transcript is expressed.

In addition, we observed that the mutant transcript is less stable than the wild-type transcript (supplementary material Fig. S1).

At the time of left-right axis formation, the mutant transcript is not subjected to NMD, predicting the production of a mutant protein during embryogenesis.

In the absence of endogenous splice junctions, only mutant transcript was detected.

To determine whether the mutant transcript was unstable, I compared RNA levels in brains of wild type and Tll2−/− mice.

Although we were unable to detect Ptc1 and Gli1 expression in the neocortex of Ptc1Lox/Lox mice by section in situ hybridisation (Fig. 1A, 1C), total Ptc1 transcript was detected by Taqman RT-PCR in microdissected VZ (Fig. 1E) and the deleted or mutant transcript was detected by SYBR Green RT-PCR (Fig. S1B).

In keeping with typical estimates for NMD efficiency (Zetoune et al., 2008), we saw ∼75 80% of mutant transcript being degraded.

In patients with NPM1 mutations, the mutant transcript was monitored by qRT-PCR in parallel with WT1, according to the method of Gorello et al (2006).