Sentence examples for mutant transcript in from inspiring English sources
The same three or six nucleotides in the regions of frameshift were interrogated for each mutant transcript in the same sequencing direction.
However, it does produce a slight reduction in the relative amount of the mutant transcript in the nucleus of KBTeloMyoD (Fig. 5B).
It should be noted that the molar abundance of the smaller mRNA appears to be lower than the wild type transcript, that is possibly indicating that there is incomplete NMD of the mutant transcript in our study.
Development of a method to achieve a sensitivity for TP53 mutation detection of at least one mutant transcript in 1000 wild-type, and ideally 1 in 10 000, requires multistep molecular techniques, as quantitative reverse-transcription PCR (QRT-PCR) cannot detect mutant TP53 against a large background of wild type.
Analysis of RNA from DM1 cell line KBTeloMyoD showed that although the mutant transcript was present in the nuclear fractions there was no indication of the mutant transcript in the cytoplasmic fractions before and after treatment with chromomycin A3 and Ro 31-8220 (Fig. 5A).
We also analyzed the mechanism whereby the present mutation caused the generation of two different mutant transcripts in more detail by determining the order of intron removal.
To get more direct evidence that Xrn2 degrades some mutant transcripts, we wanted to test whether Xrn2 binds mutant transcripts in vivo.
Sanger sequencing of synthesized cDNA confirmed the presence of both wild-type and mutant transcripts in the sample (shown in Figure 1).
We speculated that TSC1 missense mutations may cause loss of function through introduction of splicing errors in mutant transcripts in vivo.
The inclusion of cryptic exons introduced a premature termination codon, which leads to nonsense-mediated decay of the mutant transcripts in vivo.
Although similar levels of this product were observed between the WT and mutant transcripts in the chromatin fraction only WT RNA was released into the nucleoplasm.
