Sentence examples for mutant together with from inspiring English sources

Cos7 cells were transfected with Tob expression vector (WT, K63A, or W93A mutant) together with control or Flag-CNOT7 expression vector.

In this study we analysed the shoot ionome of the hma2 hma4 double mutant together with the hma2 and hma4 single mutants.

To approach this issue we transduced spleen B cells from wild type mice with retroviruses encoding AID or AIDE58Q catalytically inactive mutant, together with GFP for tracking purposes.

To test the role of REUL in RIG-I ubiquitination, RIG-I was co-expressed with wild-type REUL or the E3 ligase-defective REULΔRING (Aa 91 432) mutant together with ubiquitin.

Conjugation assays were performed by mixing S. Albany SGI1 donor strain 7205.00 with or without the helper plasmid R55 and the rifampicin resistant S. Typhimurium LT2 recipient strains (wild type or ΔthdF mutant) together with a donor-to-recipient ratio of 4 1.

The variety of Wnt-related phenotypes in the oto mutant, together with evidence that at least two distinct Wnts can be Oto-regulated, indicate that Oto plays a role in determining the timing or level of Wnt activity in multiple contexts.

(E) Cells were transfected with GST, GST-EB1 wild-type, or the indicated mutants, together with GFP, GFP-EB1 wild-type, or the mutants.

Eventually, four pairs of BRIL mutants and three pairs of Flavodoxin mutants, together with their WT proteins, were crystallized and structure determined (Tables S4 and S5).

In this study, the activities toward substrates of the M57T and M57A mutants, together with M57L mutant, were found to be much lower than those of the native and recombinant wild-type forms of CgPAD.

The ability to obtain structural and binding data for these PI3Kα mutants, together with their ATR-like inhibitor binding profiles, makes these chimeric PI3Kα proteins valuable model systems for structure-based inhibitor design.

These mutants together with prethrombin-2, the smallest zymogen form of thrombin containing only the second factor Xa cleavage site, were expressed in mammalian cells, purified to homogeneity and characterized in kinetic reactions with factor Xa in both the absence and presence of cofactors; factor Va, high affinity heparin and pentasaccharide fragment of heparin.