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The ability of the BCR-ABL triple mutant to transform primary B-lymphoid cells was assessed in Whitlock-Witte cultures.
We assessed the ability of this triple mutant to transform myeloid cell lines and induce leukemia and analyzed its ability to interact with signaling proteins and activate downstream signaling pathways.
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We designed mutants to transform secondary sites (P2, P3) into primary sites by introducing an Ala cluster in place of the native, more stable interface.
But instead of digging in, she uses her super-cool mutant powers to transform into a young man. .
These mutants failed to transform chicken embryo fibroblast cells [32].
Although we observed that the ALKM1166R mutant is unable to transform Ba/F3 cells, it was able to give rise to foci in NIH3T3 transformation assays.
Plasmids carrying genes of these EvpC mutants were used to transform an E. tarda ΔevpC mutant and their respective expression and secretion of EvpC were anlaysed.
The combined impairment of PI3-kinase and RAS activation may explain the triple mutant's inability to transform primary hematopoietic progenitor cells.
In contrast to wild-type Wip1, the phosphatase-dead mutant Wip1 (D307A) failed to transform primary fibroblasts.
The ALKF1174I mutant is able to transform both Ba/F3 and NIH3T3 cells.
In addition, we have encountered that many A. niger mutants are difficult to transform if genomic integration has been approached (e.g. ΔhacA, data not shown).
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