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The Atpht1 1 2 mutant had noticeably lower shoot Pi concentrations than WT at all time points from 150 min onwards, as might be expected from the decreased ability of this mutant to remove Pi from the nutrient solution.
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That mutant enzyme was created using a new technology called "directed evolution," which inserts mutations into the gene and selects the best mutants to remove the antigens.
Because this research worked on an individual genome of mutants, another strategy has been developed to sequence pooled DNA from different mutants to remove the effect of background mutation and increase the likelihood of identifying the causative gene.
DOI: http://dx.doi.org/10.7554/eLife.07389.006 To explore the biological functions of miR-965, independently from those of kismet, we produced targeted deletion mutants to remove the miRNA from the kismet intron.
Rabbit anti-PpdD antibody referred to as anti-HCP was a kind gift from O. Francetic (Pasteur Institute) and was used after 6× adsorptions with the EDL933 hcpA mutant strain to remove non-specific antibodies [24].
PomBase curators have begun reviewing GO annotations based on mutant phenotypes, to remove those that are known to represent indirect 'downstream' effects.
The spsC mutant failed to remove G6P from the environment, suggesting that SpsC is also an auxiliary protein as it takes part in the balance between extra and intra-cellular G6P.
CΔ19 deletion mutant was created to remove a predicted nucleic acid binding domain.
In order to understand their relevance for nucleolytic activity, the CAN2 deletion mutants were constructed to remove regions, comprising conserved residues of each domain.
The total DNAs were isolated from SN250 strain and mutants using RNase to remove RNA followed by standard phenol/chloroform extraction and ethanol precipitation.
The smc-5 (ok2421 ) mutants showed equivalent capacity to remove CPD lesions compared to wild-type, while xpc-1 mutants that are defective in GG-NER failed to remove CPDs after UV irradiation.
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