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We carried out microarray analysis of a sphinx mutant to identify possible pathways in which sphinx might be involved.
It was necessary to use a spore-colour mutant to identify a cleistothecium produced by crossing the two strains; A. nidulans is homothallic, and genetically distinct strains are much more likely to self than to cross fertilize [62], [63].
Therefore, it is necessary to screen seeds harvested from each putative mutant to identify the relatively small percentage that exhibit a heritable sis phenotype.
In this work, we have used reactivation of the NMD pathway in an NMD-defective Drosophila mutant to identify directly targeted genes.
We have used natural variation affecting the penetrance of the leaf morphology traits conferred by the semidominant Lgn-R mutant to identify a locus on the short arm of chromosome 1, sol.
We employed a non-parthenocarpic line, UC82, and the facultative parthenocarpic line, RP75/59 (pat3/pat4 mutant), to identify the genes modulated throughout carpel development and fruit set and to determine the differences between parthenocarpic and normal fruit set.
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We used these mutants to identify two separate domains of p48v-myb which had distinct roles in its accumulation in the cell nucleus.
We are using microarrays and quantitative RT-PCR using meiotic RNA from wild type and am1 mutants to identify genes that are regulated by AM1.
Since few mycobacterial proteins have so far been directly implicated in the interactions between M. tuberculosis and host cell apoptosis, we screened M. tuberculosis H37Rv transposon mutants to identify mutants that fail to inhibit cell death (FID).
Mutation-based fault localization (MBFL) is a recently proposed fault localization approach via mutation analysis, which uses the location of mutants to identify the faulty statements.
We used these mutants to identify functional genes that are potentially responsible for adhesion or invasion.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com