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We used the null background provided by the mutant to dissect the function of subdomains of the MORN1 protein.
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Thus, proteomic identification of RNA-binding regions (RBR-ID) is a powerful tool to map protein-RNA interactions and will allow rational design of mutants to dissect their function at a mechanistic level.
Transcriptomic and proteomic analyses are being performed on cells containing representative irrE tolerance mutants to dissect how transcriptional and expression profiles are reprogrammed in these cells.
Therefore, we decided to use our ΔT mutant cells to dissect the role of the CXCR4 tail in the CXCR4 signaling cascade.
For six most frequently occurring mutations, the corresponding single mutants were created to dissect the effects of these mutations.
Genetic analysis of sly and gid mutants is needed to dissect the role of GA in thermoinhibition of germination.
It will be fascinating in future studies to investigate selective mutants in vivo to dissect the relative contributions of PIP3 and IP6 in signaling.
OPT can be used to dissect mutant phenotypes and to globally map gene expression in both 2D and 3D.
The experiments in Pcl mutants thus allowed us to dissect the role of different H3-K27 methylation states in Drosophila.
Thirdly, the powerful genetic approaches and the availability of numerous mutant alleles make it possible to dissect the mechanisms underlying the function of the compounds.
Future study of intermediary metabolic flux alterations by isotopic profiling in both mutant types will be necessary to dissect whether the direction, rather than mere occurrence, of transcriptional alterations in multiple metabolic pathways is indicative of a specific adaptive response to primary mitochondrial dysfunction.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com