Sentence examples for mutant to create from inspiring English sources
This may explain the inability of ΔN141 mutant to create paracrystals with 14.5nm axial repeat.
For this reason, we first created a meg‐4 mutant in the background of the meg‐3 mutant to create the double (the two genes are closely linked on X).
An amiRNA designed to silence the ACS8 and ACS11 genes was introduced into an acs2, 4, 5, 6, 7, 9 hextuple mutant to create a line in which all eight functional ACS genes (an octuple mutant) were eliminated or reduced in function.
The identity of the correct pSET2-CΔ xf vector was confirmed by sequencing using the primers C XF F and C XF R. The pSET2-CΔ xf plasmid was then used to electrotransform the Δ xf mutant to create the complemented strain of ΔXF (designated CΔ xf) on THB agar under spectinomycin selection pressure.
To determine whether translation of Tfc6p was affected in tfc6 promoter mutants, we integrated nine copies of the myc epitope tag coding sequence onto the 3′-end of the TFC6 gene in wild type and tfc6 mutants to create carboxy-terminal 9 × -myc epitope tagged strains.
To determine whether loss of Creb3l2 function is responsible for the feelgood phenotype, we designed a Tol2-based rescue construct (Kwan et al., 2007) containing wild-type bactin2 creb3l2-mCherry that was injected into feelgood mutants to create chimeric transgenic fish overexpressing wild-type and mutant proteins (Fig. 5E).
To test whether a defective AS was responsible for the bunching at the leading edge in ush ; prdGal4 > UASush embryos, we co-expressed prdGal4 with c381Gal4 in ush mutants to create embryos with homogeneous Ush expression in the AS and striped expression in the epidermis.
The single-site C to T mutants seem to create primate-specific, predominantly highly conserved sequences because chromosomal positions of more than 90% of the examined mutant loci are conserved in the genomes of human, chimpanzee, gorilla, orangutan, and gibbon (supplementary fig. S4, Supplementary Material online).
As a tool in genetic engineering, they can be used to produce doubled haploids (recombinant inbreds) for plant breeding and gene mapping, to overcome crossing barriers (male sterility and self-incompatibility), to induce and select for mutants and to create transgenic plants.
The aim of the P2Shift and P3Shift mutant design was to create destabilizing Ala clusters at the consensus sites with minimal effect on the overall stability.
The same analysis of ΔPN197 revealed two peaks of attraction similar to Rod and ΔN197 (data not shown), yet this Rod mutant was unable to create paracrystals with 14.5nm axial repeat (Figure 3).
