Sentence examples for mutant to construct from inspiring English sources

We used this mutant to construct the shotgun library of the PaP1 genomic DNA.

The resulting plasmids were sequenced to confirm the absence of mutations and transformed into each respective mutant to construct the complemented strain.

In order to gain further insights into the origin of pathway activation in these mutants, we decided to construct double mutants deleting ROM2 or BCK1 in the gat2Δ and lea1Δ backgrounds.

We found that mutant animals failed to construct proper nests (Z = −3.4; P = 0.001) (data not shown).

Site-directed mutagenesis was used to construct mutants (Q7K, G234P, and Q7K/G234P), and the mutant, and wild-type enzymes were expressed in Escherichia coli (DE3).

We exchanged L to V at position S39 of HBsAg to construct mutant SL39V.

To facilitate the rapid assaying of protein production and purification we used PCR to construct mutant VR1-green fluorescent protein fusion genes.

Therefore, to construct mutant TCRs with high binding affinity, we need to understand the relationship between the structure and activity of these molecules.

The procedure to construct mutant hMEPE/OF45 has been described in Supplementary Figure S1.

All PCR reactions used to construct mutant strains were carried out with either Pfu DNA polymerase (Promega) or Taq DNA polymerase (NEB).

To construct mutant NLS in which the residue arginine and lysine were substituted with threonine, forward primer 5′-ACTACCACAGCCTTGACTGAGAAATGGCTTCTAGATGG-3′ and reverse primer 5′-ACTACCACAGCCTTGACTGAGAAATGGCTTCTAGATGG-3′ere used.