The E144R mutation also impairs recognition of the SUI1 AUG codon in its native, suboptimal context, and suppresses utilization of the UUG start codon in his4-301 mRNA in different Sui− mutants to confer an Ssu− phenotype.
To determine whether the difference in survival conferred by the two different alleles is the result of altered abilities to provide checkpoint function, we tested directly the ability of the mutant proteins to confer checkpoint function upon exposure to UV light or CPT.
Alanine mutations of Tyr199 on the three-stranded face of AtSgt1a-CS domain or of Thr220 on the four-stranded face, but not involved in direct contact with Hsp90, had no effect on the ability of the mutant protein to confer resistance to infection.
We modeled only the single mutants known to confer drug resistance using recently reported dose response data from single-cycle replication studies [ 46].
Interestingly, we see a general correlation between H3-L61X mutabilitybilito to confer growth on SC-his GAL medium and the degree of the defects they impart on Spt16−gene interactions as assessed by the ChIP experiments shown in Figure 3.
Previously, the afb5 mutant was reported to confer specific resistance to picolinate auxins, but no resistance to IAA, 2,4-D and dicamba [14].
Why do Smc1 M665R and Smc3 mutantmutant proteins fail to confer sister chromatid cohesion?
In agreement with the capacity of necrostatin to block IZ-induced death, the expression of RIPK3, but not a kinase dead RIPK3 mutant, was sufficient to confer sensitivity to IZ-induced death among HEY cells.
To date, mice vaccinated with attenuated type A F. tularensis mutants have failed to confer protective immunity that is better than that seen with LVS [12], [13].
Since several of the mutants that we studied (Table S1) have been described only partially or not at all, we decided to establish a phenotypic description for all the mutants in order to confer a common framework for the interpretation of the transcriptome results.
