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We also generated transgenic plants overexpressing OsBEIIa, in which a vector was introduced into the sug-h mutant to complement the phenotype.
Allelism of Staartloos with Wnt3a is therefore supported by four types of evidence: (i) genome-wide mapping; (ii) a phenotype very similar to that described in two mutant alleles of Wnt3a; (iii) a failure of the mutant to complement the Wnt3a phenotype.
The E392R ARF binding mutation also inhibits binding of EspG to PAKs in vitro (Selyunin et al., 2011) and therefore the inability of this mutant to complement Δ espG may be due to a combination of these factors.
The failure of the BubR1 gain-of-function mutant to complement the depletion of wild-type BubR1 strongly suggests that the evolutionary divergence of Bub1 and BubR1, with its specific effects on kinetochore recruitment, is functionally relevant.
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The ability of this group of mutants to complement infection showed a similar pattern; with the exception of K176A, which successfully complemented infection, all of those tested were classified as being resistant-like (R).
The ability of these mutants to complement Δ espG in functional assays was tested.
The identity of the cpr5 try-JC double mutants was confirmed by the failure of the double mutants to complement either parent mutant after crossing.
When we measured the ability of wild type PIN3 and mutant PIN3 S4A S5A to complement the pin347 mutant when expressed from a PIN3 promoter fragment, we found that the phototropism defect of the pin347 mutant was only partially complemented by the PIN3 S4A S5A transgene while it was fully complemented by wild type PIN3.
For two mutants, or990 ts and or1247 ts, a different mis-sense mutation was found in the same gene in both mutants; this was the only candidate gene shared by both mutants, and the two mutants failed to complement each other (Table S1).
We have added mutant analysis, to complement morpholino data, wherever possible.
All three cals5 exine defects were fully recessive (e.g. Fig. 1l), and pair-wise crosses between each mutant failed to complement.
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