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We determined a 'cheating' mutant to be a non-producer which (1) had lower fitness (grew less well) than the producer genotype in monoculture, and (2) gained a growth advantage in mixed culture, such that it grew as well or better than the wild type, that is, the mutant was maintained in the population and may have increased in frequency.
Because the association between the A1 chain of CT and other components of the retro-translocation machinery is likely transient or very weak, the successful bioengineering of such a mutant to be trapped as an intermediate in ER is essential for affinity isolation and further analysis.
That condition is specified in lines 3 though 5, which require the proficiency score of a passing mutant to be at least 0.45.
A similar strategy has allowed causal lesions in a different C. elegans mutant to be identified as well (G. Oikonomou and S. Shaham, unpublished observations).
We considered a mutant to be a candidate hit if it elicited fluorescence levels differing from this average response to a statistically robust extent (see below).
Relative to the parental strain, all Ins strains under-accumulated 18S rRNA, while the levels of 25S rRNA did not seem to be affected, proving our screen based on an rps mutant to be small subunit specific.
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N is the library size of mutants to be screened in a single generation.
Moreover, investigation of mitochondrial respiration by in vivo 31P nuclear magnetic resonance spectroscopy showed the tpk1- and not tpk3-mutant, to be defective in glucose repression [ 24].
Some holdout mutants appear to be battling a rearguard guerrilla action against terrifying new soldier-robots called Sentinels.
Growth of the had1∆ mutant was found to be compromised at 38° or higher.
Furthermore, the Ghd10 locus of the ghd10 mutant was shown to be allelic to Ehd2/RID1/OsID1.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com