Sentence examples for mutant thereof from inspiring English sources

Here we describe a protocol for expression and purification of mature human VEGF-D, and a mutant thereof with reduced glycosylation, potentially suitable for preclinical therapeutic and structural studies, respectively.

Briefly, E. coli TUNER DE3) was transformed with either plasmids encoding the C-terminal domain of Phl p 1.0102 or the mutant thereof.

The assay is based on the use of two oxidases: wild-type chito-oligosaccharide oxidase, ChitO, and a mutant thereof, ChitO-Q268R.

Co-IP using anti-C/EBPα antibodies resulted in precipitation of TBP by extended-C/EBPα, as well as the S299D and the S299D-ΔNoLS mutant thereof.

This could be circumvented with the SLAC1 phospho-site mutants S59, S120 and a double mutant thereof, introduced into SLAC1 loss-of-function mutants.

We observed that overexpression of both extended-C/EBPα and the S299D mutant thereof strongly induced RNA Pol I-mediated transcription of the reporter RNA to a similar extent as c-Myc, which was used as a positive control.

The CueO laccase and mutants thereof were capable of oxidizing benzopyrene (Fig. 7a, b).

The crystal structures of D3.4 and mutants thereof show how high specificity and inhibition is achieved.

To assay polymerase complex formation, HEK293T cell cultures were co-transfected with plasmids pCPB1, pCPB2 (or mutants thereof) and pCHisTEVPA.

Escherichia coli strain DH5α was used for routine cloning and S. typhimurium strain SL1344 and isogenic mutants thereof, referred to by genotype, were used in this study.

To determine the RNP biological activity (transcription plus replication), HEK293T cells were co-transfected with plasmids pCPB1, pCPB2 (or mutants thereof), pCPA, pCMVNP and pHHNSCAT as indicated.