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Only PNPase deletion resulted in more up-regulated (~59%) than down-regulated transcripts (~37%), but even in the ∆ pnp mutant there were still a considerable percentage of down-regulated transcripts (Table 1).
At the V stage, transcripts of 139 genes were present in higher amounts in the gln1.3 mutant as compared to the WT (Additional file 1: Table S6b), whereas in the gln1.4 mutant there were only 54 (Additional file 1: Table S8b).
As for the PNPase mutant there were 226 differentially expressed transcripts (Additional file 1: Table S3).
In hyphae of the chs2Δ chs8Δ mutant, there were increased proportions of dead short hyphae and dead end compartments compared to wild-type (Table 3).
In the RNase II mutant there were 187 transcripts differentially expressed when compared with the wild-type control (Additional file 1: Table S1).
All four models generated a double map for the Isl2-EphA3ki/ki muthere there were, however, subtle differences between the model results and experimental data.
Similar(48)
In the library of 100 random mutants there were 49 independent mutants detected with a total count of 102 recurrent mutations, which is a higher proportion of recurrent mutations than was expected [ 5].
And although many of these are harmless, amongst the myriad mutants there are those whose effects are anything but benign: subtypes 6 and 11 can lead to genital warts; subtypes 16 and 18 (amongst others) can lead to cervical, vulvar, vaginal, penile, anal and oral cancers.
For both mutants there was altered expression of genes involved in nucleotide biosynthesis at the non-permissive temperature.
In addition, in brm mutants there is an increase in the amount of H3K4me3 at the FLC locus, as was previously shown for the fve and fld mutants [27], [60].
In the mutants there is a strong bias for UNC-40 GFP UNC-40 GFPocalized to the dorsal region.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com