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A fire would not have caused as much panic as that flying mutant: there was no telling what she might do.
Even in 4A mutant, there was some residual phosphorylation, indicating that other kinase(s) also phosphorylate SIRT1 in vivo.
In the wildtype and dnaA mutant, there was no difference in the steady-state level of transcripts from the dnaC operon at high and low temperatures.
In the ΔchpBIK mutant, there was a reduction in cell death only after treatment by DNA damaging agents (Figure 2C,D).
Although two genes (recR and uvrB) also showed increased transcription at 42°C compared to 30°C in the dnaA46 mutant, there was no coordinated response of the SOS regulon in this strain.
However, we found that in a rab-10 uev-1 double mutant, there was a significant increase in GLR-1 accretion size and number only compared to the rab-10 single mutant, but not compared to the uev-1 single mutant alone (Figure 7G, H, K, L).
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For both mutants there was altered expression of genes involved in nucleotide biosynthesis at the non-permissive temperature.
And although many of these are harmless, amongst the myriad mutants there are those whose effects are anything but benign: subtypes 6 and 11 can lead to genital warts; subtypes 16 and 18 (amongst others) can lead to cervical, vulvar, vaginal, penile, anal and oral cancers.
In addition, in brm mutants there is an increase in the amount of H3K4me3 at the FLC locus, as was previously shown for the fve and fld mutants [27], [60].
In the mutants there is a strong bias for UNC-40 GFP UNC-40 GFPocalized to the dorsal region.
In the library of 100 random mutants there were 49 independent mutants detected with a total count of 102 recurrent mutations, which is a higher proportion of recurrent mutations than was expected [ 5].
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CEO of Professional Science Editing for Scientists @ prosciediting.com