To test the function of AtELP3 in the presence of NPR1, Atelp3-10 npr1 was crossed with wild type, and Atelp3-10 single mutants were identified in the segregating F2 population based on their morphology (same as the Atelp3-10 npr1 double mutant) and confirmed by the Atelp3-10 anpr1pr1 dCAPS markers (Additional file 1: Table S1).
Double soh715hsr8-1 mutants comprised 1/4th of the segregating population instead of the expected 1/16th.
We have added in the following material to the Discussion: "We should also note that even with all of our efforts to clean up the respective backgrounds and validate that the mutant phenotypes are similar to the segregating natural genotypes, it remains possible that some of the observed effects are caused by unexpected changes in the lines".
We should also note that even with all of our efforts to clean up the respective backgrounds and validate that the mutant phenotypes are similar to the segregating natural genotypes, it remains possible that some of the observed effects are caused by unexpected changes in the lines.
Double and triple mutants were identified by PCR genotyping of the segregating F2 population.
Each of these F3 families is fixed (homozygous) for one of the mutant loci, but segregating the other.
Ntl129A/Cy stocks carrying the P insertions are stable, with fertility of homozygous mutant males segregating consistently with the w+ marker of the P element.
In the original spore selection, the mutant allele segregated at only 91%, the low end of our threshold, and the mutation changed a poorly conserved isoleucine to leucine, suggesting it is unlikely to have a major effect on the protein's activity.
The double mutants segregating in the progeny of rps10b-1 pid-14/+ plants had a more severe phenotype than pid-14 alone, as neither cauline leaves nor branches, nor flowers were produced on the primary inflorescence, and the proportion of empty rosette axils was increased.
The two mutant lines segregated for the expected brown midrib (bmr) phenotype, a trait associated with altered lignin content and increased digestibility.
