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In the case of replacements where both clusters go extinct (the resident being extinct before the mutant) the mutant cluster re-invades first.
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Compared with the wild type and other mutants, the mutant R194A showed the highest catalytic efficiency (kcat/Km), which was more than three-times that of the wild type.
Among these mutants, the mutant T16A/T39I/L176Q showed highest stability at 70 °C and retained 45.9% of its activity after 60 min of incubation while the wild type had lost its activity completely after 50 min of incubation.
For example, in a sample containing 50% tumor cells, provided that all of them were KRAS-mutant, the mutant alleles to be amplified would correspond to 25% of all alleles included in the sample, which is below the limit of heterozygous calling by the SDS software.
After screening for mutants, the mutant DNA was sequenced and transformed into BL21 cells.
For ten of the mutants, the mutant phenotype is recapitulated in classical or gene deletion mutants in Neurospora or other filamentous fungi.
After screening several mutants, the mutants Gln277Ala and Phe554Ala have been found to be more thermostable than the wild-type PA.
In the case of mutant G109C/Basal mutant, the inserted amino acid is close to a conserved disulfide bond (Cys110 Cys187) in rhodopsin.
For mutant E113C/Basal mutant, the λmax of its chromophore was 380 nm.
The cysteine mutant E113C/Basal mutant showed the fastest reaction rate with 4-PDS among all of the mutants at helix-III.
The single-cysteine mutant G121C/Basal mutant (t1/2, 1.8 min) showed the fastest Meta II decay among rhodopsin mutants (Table 1).
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CEO of Professional Science Editing for Scientists @ prosciediting.com