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After 1 h, only few cells were found at the bottom of a 96-well plate in both, the wild-type and the cheR1 mutant, the majority of which was non-motile.
Instead, in the RHB mutant the majority of these co-regulation clusters was broken, with the exception of ccd4 and chy-b genes which remained co-regulated (Additional File 3C).
In yeast cells of the chs2Δ chs8Δ mutant, the majority of PI-positive stained cells were dead small buds, indicating that death occurred during the very early stages of polarized bud growth (Table 3).
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Consistent with these findings, western blotting showed that SRF protein levels were 80% lower in mutant compared to control skeletal muscles (Fig. 1A) and in mutants the majority of the myonuclei were no longer immunostained for SRF (Fig 1B).
In the case of K650M and K650E mutants, the majority of STAT1 activation in cells is likely a result of direct phosphorylation and may result from intracellular activation [22].
However, in ap, lms double mutants the majority of LT muscles are still present as well, thus ruling out that the two genes are required for LT muscle specification in a mutually-redundant fashion.
In ipla-1 mutants, the majority of seam cells underwent the S4 divisions in various directions (52%, n=107; Figure 2H).
In Drosophila Perlecan (dPerlecan) loss-of-function mutants, the majority of neuroblasts appear to increase in size but then remain G1 arrested (Datta, 1995).
In both photoreceptor mutants the majority of genes was downregulated in light (769 in Δ blr1 and 873 in Δ blr2) and exert diverse functions.
For the resulting mutants, the majority of the coding region of each gene was replaced by the kan or cat gene, except for the first and last 50 nt.
When Ret MEN2B was overexpressed in the background of Pink1 mutants, the majority of flies showed significantly improved muscle morphology, with only 12% of flies displaying degenerated myofibrils (Fig 1D and L).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com