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In Foxd1 −/− mutant, the expression of Isl2 reduces dramatically and Isl2 inhibits EphB1 and Zic2.
In this mutant, the expression of PkcA was repressed by glucose, but induced by glycerol (Ronen et al., 2007).
In order to further confirm whether the defense responses were activated in spl5 mutant, the expression of OsPR1a, a marker gene of systemic acquired resistance (Durrant and Dong, 2004), was tested by our Real-time PCR (Figure 3).
As shown in Figure 3b, in spl5 mutant, the expression of 6 genes were induced and 2 genes were suppressed, and 6 genes did not changed at mRNA level.
These results indicate that while expression of the chromosomal copy of iPGM alone is not sufficient to fully compensate for the lack of dPGM activity in the ΔdPGM mutant, the expression of additional iPGM from a medium copy plasmid can restore the mutant cells to normal growth characteristics.
Furthermore, this result supports our conclusion that RpoN would be directly required for hcp gene expression since in the ΔrpoN mutant the expression of Hcp was totally abolished despite the fact that in this mutant the level of HapR was increased.
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In dfoxo Δ94 mutants, the expression of all three MNC-expressed dilps was significantly reduced compared with control flies (Fig. 8A).
In these mutants, the expression of several OsPIN genes is altered.
In nal2/nal3 mutants, the expression of different OsPIN genes is modified.
To minimize the possibility of selection of the undesired expression-improved mutants, the expression level of wildtype Rubisco was firstly maximized in E. coli through overexpressing the Rubisco-specific molecular chaperone and optimizing the expression conditions.
(C E) Compared with lin-35 (C) and lsy-2 (D) single mutants, the expression level of GFP PGL-1 is dramatically increased in lin-35; lsy-2(RNAi) double mutants (E).
More suggestions(15)
variant the expression
variants the expression
mutations the expression
transformants the expression
mutant the fluorescence
mutant the lack
mutant the bias
mutant the position
mutant the differentiation
mutant the increase
mutant the histidine
mutant the rpoB
mutant the conclusion
mutant the elongation
mutant the accumulation
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