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In the pc mutant, the corresponding time was significantly longer (348.0±164.9 s).
For an IDM- lytA mutant, the corresponding values were 8.5 days versus 2 days (p = 0.019), respectively, for non-supplemented medium, and 2.5 versus 2 days (p = 0.021), respectively, in the presence of cat.
In the present study we have observed that in experiments using the endothelium specific CD73 mutant, the corresponding loxP controls showed a stronger tumor growth and edema formation when compared to WT controls of the global CD73 knockout.
As with the Rsp5WW1-3-HECT D495A mutant, the corresponding D584A and D639A mutant versions of NEDD4WW1-4-HECT aNEDD4LWW1,3-4-HECT NEDD4LWW1,3-4-HECT NEDD4LWW1,3-4-HECTte.
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The M44L mutant and the corresponding prodomain deletion mutant (ΔN-M44L), but not M27L and M39L, inhibited casapase-3 activation as compared with the activations of the wild-type and ΔN mutant, respectively.
While a few designed siRNAs targeting the mutants appeared to discriminate between the mutant and the corresponding wild-type alleles to some degree, most of the siRNAs resulted in weak gene silencing.
A single mutation, P159A, was found to be responsible for most of the enhanced solubility observed in the six mutants, and the corresponding single mutant also retained the hydroxylation activity.
To generate the RyR2 mutants G1885E, G1886S, G1886D, and the double mutant G1885E/G1886S, the corresponding SNPs were introduced into the full-length mouse RyR2 cDNA using the overlap extension PCR method as described by Ho et al. (14).
The results of this epistasis analysis were further confirmed by scoring the average number of induced VPCs in the single Muv mutants and the corresponding double mutants (Table 2).
One week old plants were transplanted into pots with 1 1 peat:vermiculate mixture with one Col-0, one cat2, one of the single mutants and the corresponding cat2 double mutant (4 plants per pot).
The mutant and the corresponding wild type fragments expressed in Escherichia coli are refolded into functional conformations only in the presence of S-peptide.
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