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Thus, we next investigated the AMT conditions using germinated conidia from the pyrG mutant that were pregrown for 24 h.
The binding analysis using Biacore showed the association and dissociation parameters of K46E mutant that were optimized with IgM.
Work from Dong, et al. described the results from a knock-in mouse expressing NF-κB S276A mutant that were embryonic lethal [32].
A transient antitumor effect was also observed in tumor-bearing C/L858R mice (that express the erlotinib-sensitive EGFR mutant) that were similarly treated with 17-AAG (data not shown).
We further analyzed the dephosphorylation of CDK9's Ser175 by utilizing WT CDK9 and CDK9 S175A mutant that were expressed as FLAG-tagged constructs in cultured cells, labeled with (32P) orthophosphate in the presence of okadaic acid and subjected to immunoprecipitation and dephosphorylation by PP1.
For an in vitro kinase assay, the recombinant His-fusion NTD RSK2 (residues 44 367) wildtype and K216A mutant that were purified on a HiLoad 16/60 Superdex-200 column at a protein concentration of 0.5 0.7 mg/ml without the addition of AMP-PNP.
Similar(49)
Brucella abortus vaccine strain RB51 is an attenuated, stable rough mutant that is being used in many countries to control bovine brucellosis.
A mutant that is either a doublet or a multiplet.
Interestingly, these two characteristics were also found for the m4 mutant that is slightly toxic.
The 3xA FGF1 mutant that is defective in FGFR binding site [12] was used as a control.
We used the constitutively-active RanQ69L mutant that is deficient in the ability to hydrolyze GTP [62].
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