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Moreover, we designed a SOX2 mutant that we predicted and confirmed experimentally to bind cooperatively with OCT4 to the compressed motif.
In both the wild type pro-VEGF-C and the cleavage-resistant mutant used by Bui et al., the C-terminus is still attached to the protein, whereas it is absent in the deletion mutant that we analyzed in vivo and in vitro.
We also compared the radiance signal from animals infected with a wild type strain and a Δcaf1ΔpsaA mutant that we previously showed to be attenuated in colonization of the lymph node and systemic dissemination.
In summary, Tex19.1−/− mouse mutant that we generated confirms the spermatogenic phenotypes of a previously reported Tex19.1 mutant [15].
Conversely, the only S769N mutant that we observed was found in a fully susceptible isolate.
Based on our structures, we additionally designed a R81A/Y87F double mutant that we expected to have the strongest effect.
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Among the 23 mutants that we constructed, the most promising Y216W showed an 18 ± 2.7% increase in specific activity by comparison with the wild type enzyme.
Heck, even if GDP shrank an impossible-to-imagine 10percentthathat would take us back to the dire wasteland of warlords and mutants that we last suffered through in [checks notes] er, 2013, which didn't seem like such a dystopia at the time.
In addition, 8 mutants that we detected were found in different ethnic backgrounds.
The observation that Δprocyclin is notably more robust than other procyclin mutants that we have generated previously is most probably because of the experimental strategy that was used.
This observation suggests that, early in the period of evolution, the cheating mutants that we see are those that are able to gain a foothold in the population from an initially low frequency.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com