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We mutated Cys26 to Ala and also made a mutant that replaced Cys74 and Gly75 with alanines.
We also created a mutant that replaced these same two residues with aspartic acid, creating a phosphomimetic APC2 (APC2DD).
We also created a mutant that replaced these same two residues with aspartic acid, creating a phosphomimetic APC2 (APC2DD)." We also added additional annotations to Figure 8B, making all of this clearer.
We thus focused on the least altered of these, the mutant that replaced the more N-terminal two serine residues with alanine (APC2AA; Figure 8B, red arrows), thus preventing phosphorylation.
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The physical mechanisms that modulate the coupling (local cooperativity) among amino acid residues on the extensive binding surface of RBD1 are investigated here, using mutants that replace a highly conserved glycine residue.
All HSV-2 ICP0− mutant viruses bore a common deletion that replaced codons 19 to 104 of the ICP0 gene with a green fluorescent protein (GFP) coding sequence.
The genetically engineered NA-deficient mutant also protected animals against the challenge and induced humoral immune response against the foreign protein that replaced the NA.
— that replaced it.
By site-directed mutagenesis we constructed a MAG mutant that had Arg118 replaced with Ala, termed MAG R118A (Fig. 6A).
For our studies, we also considered a lysine mutant, TMVLys, which displays a genetically introduced lysine residue that replaces threonine at amino acid position 158.
Pronouns are words that replace a noun.
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