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Based on this rationale, we were able to successfully generate a C. glutamicum mutant that produced relatively high levels of GABA.
Recent data from chloroplasts, involving the mitochondrial ATP synthase gene (atpB), demonstrated that a chloroplast mutant that produced a complementary coded atpb NAT mRNA resulted in dsRNA formation, which prevented exonuclease mediated degradation of the atpB mRNA [ 39].
Previously, app was found to be down-regulated in mutants with a block at the stage of young fruiting bodies (protoperithecia), but normal in a developmental mutant that produced fruiting bodies, but no mature spores (Nowrousian et al. 2007b).
In addition, a Tn5 mutation in the promoter region of F. tularensis SCHU S4 between open reading frames FTT0673-0674 resulted in a mutant that produced a capsule but the mutant does not express a full length O-antigen.
3MB was quantified after 24 and 48 h, and the mutant that produced the greatest amount of 3MB was chosen for further analysis.
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Using a GBS mutant that produces adhesin-less pili, it has been shown that the pilus backbone itself promotes phagocyte resistance and systemic virulence [25].
Analysis of a separate RNAi mutant that produces cells with a short unconnected new flagellum also lacking a new FAZ revealed that the basal body was localised at the far posterior end of the cell (M.B. & D.R., unpublished data).
The morphological marker was a recessive mutant that produces a dumpy phenotype.
In the early 1970s, an immature fiber (im) mutant that produces non-fluffy bolls with low MIC fibers was discovered [ 9].
We also found that vit mRNA levels were low in a feminized mutant unable to produce sperm, but were normal in a masculinized mutant that produces sperm but not oocytes.
We compared the titration of the wild-type complex, which mediates an attractant single-photon response, to that of the SRI−HtrI_E56Q complex, an inverted mutant that produces a strong repellent single-photon response.
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