We show that Ku's interaction with a TRF2 mutant that induces telomeric fusions is significantly impaired.
To map the active conformation of Snf7, we reconstituted spin-labeled full-length Snf7R52E polymers on lipid membranes, where R52E is a previously characterized activation mutant that induces Snf7 polymerization (Henne et al., 2012).
Moreover, it may be possible to bias repair to HR over NHEJ by exploiting a Cas9 mutant that induces single-strand DNA breaks (Jinek et al. 2012; Cong et al. 2013; Mali et al. 2013) or conducting Cas9-mediated genome engineering in a genetic background that reduces NHEJ (Beumer et al. 2008).
In support of this idea, it was previously described that NMJ defects similar to TBPH loss of function were found in several other mutants that induce alterations in the organization of the microtubules (MT) at the synaptic terminals [24], [25].
Utilizing various mutants that induce melanotic capsule formation and also a real immune challenge provided by parasitic wasps, we show that the acute expression of the ADGF-A protein is not driven by one specific signaling cascade but is rather associated with the behavior of immune cells during the general inflammatory response.
This effect was observed in a previous study of PAH mutants that induce PKU and other studies using analogues of the substrate or cofactor.
From our previous study on RNA biogenesis mutants that induce hybrids, we noted that many of these mutants allow cryptic transcription, and likely the production of aberrant transcripts (Wyers et al., 2005; Cheung et al., 2008; Wahba et al., 2011).
This possibility is further strengthened by the recent finding that SmpB binding protects U85 from chemical modification and that this protection shifts by one nucleotide in tmRNA mutants that induce misreading of the resume codon [ 24].
In a genetic screen to identify Listeria monocytogenes mutants that induced altered levels of host cell death, we identified a mutation in lmo2473 that caused hyperstimulation of IL-1β secretion and pyroptosis following bacteriolysis in the macrophage cytosol.
Applying a cut-off of SR >2.0 or <−2.0 yielded 2712 unique transposon mutants that induced altered fluorescence in one or more of the 12 IR clones (Figure 2A B).
Mutant mycobacteria strains that induce poor granuloma formation result in increased bacterial growth [5] [9].
