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By using this system, we have distinguished the mutant that has one base substitution from the fully complementary target.
This conclusion is supported by the crystal structure of another mutant that has two hydrophobic residues substituted for the two non-hydrophobic residues.
When SLs are exogenously applied to the rice d10-1 muthat thas has a highly branched phenotype caused by a defect in the SL biosynthesis gene (CCD8), they inhibit tiller bud outgrowth (branching in rice) of the mutant.
The 10th variant is a simple deletion mutant that has lost the sequences between 32 and 12 kbp from the right-hand end of the genome.
The effect of overexpressing the bifunctional enzyme was studied in FAO cells transduced with recombinant adenoviral constructs of either the wild-type enzyme or a double mutant that has no bisphosphatase activity or protein kinase phosphorylation site.
Similarly, a T-DNA activation-tagged mutant that has OsMADS45 activated and expressed in leaves and other tissues revealed early flowering and the same early flowering phenotype was observed by overexpressing OsMADS45 in a Taiwan rice variety TNG67.
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Studies with a TR mutant that had slightly decreased T3-binding affinity (R320H) showed a T3-dependent decrease in binding to N-CoR and increase in binding to SRC-1 that reflected its decreased ligand binding affinity.
The only gene deletion mutant that had a significant increase in TEV protease production was ubc4Δ.
By site-directed mutagenesis we constructed a MAG mutant that had Arg118 replaced with Ala, termed MAG R118A (Fig. 6A).
The two mutants below 30 g had shortened lifespans of less than 700 days, as did the one mutant that had a maximal body weight above 35 g.
Depletion of p53 has been shown to rescue development in a Tcof mutant that had reduced ribosome function (24).
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