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So I took a yeast mutant that couldn't divide because it was defective in the controlling gene.
In this study, we attempted to create a lysine-deficient LIGHT mutant that could be PEGylated site-specifically and would have lower affinity for DcR3.
A Zfp277 mutant that could not bind Bmi1 did not rescue Zfp277−/− MEFs from premature senescence.
In order to further understand the regulation of WRN acetylation and its stability, we identified WRN acetylation sites and made a WRN mutant that could not be acetylated to test its stability.
We are also in the process of defining the shortest deletion mutant that could confer resistance to DT and ETA: preliminary results indicate that the same human Dph2 deletion mutant is functional but not a shorter version with a 133 amino acid deletion (data not shown).
An HS1 tyrosine mutant that could not translocate to the nucleus, also failed to induce apoptosis [ 37].
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From this screening, we isolated 24 suppressor mutants that could grow on 5-FOA medium.
With reckless ambition, I decided to add this fusion back into cells and then screen for mutants that could not either switch on or switch off the response to heat shock.
Various characters were included at the suggestion of editor Mark Helfrich, who brought to director Brett Ratner Marvel's X-Men Encyclopedia searching for mutants that could make appearances.
Similar observations were made with NTS1 directly after solubilization [16] and with structurally unstable constitutively active receptor mutants that could be stabilized by agonist addition [17] [19].
Therefore, a vaccine based upon sequences that are naturally highly conserved in all influenza A viruses may greatly restrict the range of possible mutants that could selectively overcome immune suppression.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com