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Interestingly, the mutant that converts Glu27 to Val, i.e. the residue found in all the other NPs (NP7(E27V)), demonstrated that the heme orientation was reversed from A to B, whereas the replacement of Glu27 by Gln, NP7(E27Q), did not change the heme orientation compared to the wild type, and replacement of Val24 by Glu in NP2, NP2 V24E), resulted in the B → A reorientation of the cofactor.
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Using previously established stabilization strategies, we engineered a stabilized E113Q counterion mutant that converted rhodopsin to a UV-absorbing photoreceptor with deprotonated Schiff base and allowed reconstitution into native-like lipid membranes.
Sequencing of the PCR products identified a G to A change in the mutant pool that converts a glycine to an arginine at position 484 (Figure 1H, J) in the catalytic domain of Aldh1a2 (Figure 1K).
But in a recent issue of Proceedings of the National Academy of Sciences, Dr Duesberg argues that nobody has yet identified the mutant genes that convert a normal human cell into a cancerous cell.
In Arabidopsis, recent experiments with mutants impaired in the trans-hyroxylation step that converts iP to tZ have revealed that the regulation of cell proliferation in the shoot apical meristem is a function exclusive to tZ [ 71].
The vtc1-1 mutant contains a point mutation in amino acid 22 that converts a conserved proline into a serine.
Furthermore, the accumulation of aldehydes was also consistent with the deficiency of the CER1 step that converts aldehyde to alkandes in the GL mutant.
In a previous report [ 25], we described a new neuroanatomical phenotype in Bbs2 −/−, Bbs4 −/−, Bbs6 −/− and Bbs1 M390R/M390R knockin mutant mice (mice homozygous for the most common human BBS mutation that converts a methionine codon to an arginine codon).
Since acetate kinase is the enzyme that converts acetyl phosphate to acetate and ATP, an ackA mutant is also expected to be defective in anaerobic growth on xylose.
Consistent with mRNA changes, the level of thiamine pyrophosphate (TPP) and TPP-utilizing pyruvate decarboxylase activity that converts pyruvate to acetaldehyde were also reduced in the mutant.
In fact, a single-point mutation that converts the glycine residue into cysteine was sufficient to generate a mutant protein with activity similar to a bona fide DSP [ 7].
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