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At late larval development, ball mutant testes contained cysts at progressive stages of proliferation, including mature 16 cell cysts as in the case of wild type larval testes.
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Furthermore, although initially present, SSCs are reportedly depleted by 8 10 weeks of age, resulting in mutant testes containing seminiferous tubules completely devoid of germ cells (Hata et al., 2006).
Mutant testes also contain low levels of a normally spliced product, suggesting that the mutation is an extreme hypomorph and not a complete null.
Overall, Cyp26b1SC−/SC− testes contained fewer germ cells in comparison with Cyp26b1SC+/SC+ testes (Figure 4).
These testes contained spermatogonia A, spermatogonia B, and spermatocytes but no spermatozoa.
Two of 10 scrotal testes contained similar abnormalities, although spermatogenesis was ongoing.
Moreover, the miR-dKO testes contained thinner seminiferous epithelia and larger lumens, as compared to WT control and single KO testes at 10 weeks of age (Fig. 2).
The expression of DAZAP1 increased afterward, as the testes contained increasing number of proliferating and meiotic germ cells.
Histological analysis revealed that KI/KO and wild-type testes contained the full spectrum of spermatogenic cells including mature spermatozoa, whereas Tex11-null testes exhibited meiotic arrest of germ cells as previously reported (Fig 2E) (Yang et al, 2008).
As testes contain various cell types including developing germ cells, the testicular transcriptome is complex with many yet uncharacterized transcripts.
As those testes contain mostly meiotic cells, MSCI was ruled out as a possible process [ 32].
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