Sentence examples for mutant templates based from inspiring English sources

Some details underscoring the challenges: 1) The inherent nature of mosaicism is that a population consists of a complex mixture of templates consisting predominantly of germline sequences intermixed with myriad mutant templates (based upon data in our manuscript showing diverse APP copies by qPCR and PNA FISH indicative of diverse genomic structures).

In each group, the priorities are assigned to templates according to the hierarchical classification of templates based on their complexity.

Fig. 4 Template based approach for meta-workflow creation Fig. 5 Template based meta-workflow creation.

Her vision is a novel economic and legal template based on crowdfunding and power-sharing.

Structure modelling can broadly be divided into template-free and template based methods (Moult et al., 2009).

Based on Poisson distribution, the expected average number of mutant templates per reaction is estimated using a formula (Poisson distribution) f(0) = e−x, where x is the average number of mutants per reaction [7].

Based on the above, we developed the following scheme to decode the interaction potential: Build homology submodels of mutant TF based on templates from known complex structures.

Every positive reaction is regarded as being derived from one or more copies of mutant templates.

Templates for in vitro transcription were made from wild-type and mutant templates by PCR using Pfu DNA polymerase (Fermentas) and oligos C287: 5′-AATTTAATACGACTCACTATAGGGTTGGGAAGTATCAT and C288: 5′-TCACCATGGTTGTTGAAGTGCACAGATTG.

Finally, the '2 × 2' oligonucleotide produced a two base mismatch on either side of the mutation site in the wild-type sequence, but produced a five base mismatch (the mutation site plus two base positions either side of it) in the mutant template.

An oligonucleotide designated '1 × 1' produced a single base mismatch when bound to the wild-type sequence, but produced a two base mismatch when bound to the mutant template (i.e., at the mutation position and one position adjacent to it).