Sentence examples for mutant subclones during from inspiring English sources

The multifaceted activities of XPO1 inhibitors may not only enhance p53-mediated MCL cell death but also delay or prevent the selection of p53 mutant subclones during therapy.

(37) Clinical concern about p53 activators would be the selection or induction of p53 mutant subclones during therapy, which has been reported in in vitro cultures with Nutlin-3a.

Slow growing mutant subclones were observed.

Cytogenetic analysis was performed on the HPRT-mutant subclones selected from unirradiated Chinese hamster V-79 cells and from HPRT- mutant subclones that arose after exposure to γ-rays, 1GeV protons and 14N-ions (LET - 77 keV/μm), produced by the synchrophasotron and the U-400M heavy ion accelerator.

Of the initially characterized subclones (column one of Table 2), all of the 28 singly mutant cells formed light blue colonies on rich medium with X-gal, while the 16 doubly mutant subclones formed dark blue colonies.

Additional primers were designed, as needed, for linking plasmid subclones during the compilation of the initial genome sequences, and for sequencing PCR products and plasmids.

Stably transduced cell lines were subcloned during the course of antibiotic selection.

The behavior of these systems, could also be explained if one common small-effect mutant, formed during nonselective colony growth, initiated a subclone within which rarer secondary mutations lead to the observed Lac+ revertant (without mutagenesis).

Wildtype βS, αS and its disease associated mutants were subcloned into pcDNA plasmid transfected using FuGene 6 transfection reagent (Roche applied science).

The resulting mutants were subcloned into the pEGFP-N1 vector for binding and localization studies.

The generated Flag-tagged mutants were subcloned in both pMK33 and pCasperhs83 vectors as previously described [ 35].