Both NKX2-5 mutantstudieded also recognised a large number of targets uniquely (C-set), which we term off-targets.
Mutant studies would also be useful in assessing the physiology of early exponential growth phase aerobic Z. mobilis cells.
In double mutant studies, we also determined that while loss of Gria3 alone does not result in SWD, its presence is nevertheless associated with the high incidence of SWD in mice homozygous for the Gria4 spkw1 allele.
Our studies also show that the ΔBB mutant of NF2, like another commonly studied "Blue Box" mutant, Merlin BBA, is in fact tumorigenic in mice.
In addition, previous studies also have verified that mutants LATS1-K734M/LATS2-K697A/mLats2-K655M are all kinase-dead (Zhao et al., 2007; Wei et al., 2007; Visser and Yang, 2010).
However, these studies also showed that bona fide CRC-driver mutations are often outcompeted by non-mutant ISCs.
Interesting to note is that the mutant obtained from this study also overexpressed eight oxioreductases that can use NADPH as an electron donor.
The heterozygous mutants of the present study also display notable differences from Foxp2-KO heterozygotes, which were reported to show moderate developmental delays and abnormal cerebellar histoarchitecture, including retainment of a one-cell-thick EGL at P17 [26].
The ISP mutants characterized in the current study also have similar phenotypes to the L. major kinesin mutants, which have shortened flagella upon overexpression of kinesin, while comparable siRNA knock-down mutants of the equivalent T. brucei gene have elongated flagella due to the micro-tubule binding and depolymerizing activity of kinesin (Blaineau et al., 2007).
Several of the mutant lines examined in this study also possessed very low levels of trienoic fatty acids but were accompanied by distinct alterations in other fatty acids.
