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SL1 had the most active TOR of the three mutant strains, supporting the importance of the RQD helix.
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This set of molecular tools enables an in-depth phenotypic analysis of Z. tritici mutant strains thereby supporting new strategies towards the goal of controlling wheat against Z. tritici.
Inoculation of the ecsA::intron mutant and wild-type Newman strains supported the results obtained in the infection with ΔecsAB mutant and wild-type LS-1 strains with respect to weight loss and frequency of arthritis (data not shown).
Colonization with SC5314 was equivalent to that of CAI4, the parental strain from which the mutant strains were derived (Supporting Information Fig. S1B).
Results obtained for the site-directed mutants of Met6 indicate that less than 30%% residual enzyme activity is not sufficient to support growth of mutant strains in Met media.
In contrast to niaD or nirA mutants strains lacking nrtA and nrtB function are only moderately chlorate resistant, and high concentrations lead to strong toxicity whereas lower chlorate concentrations support residual growth of the mutant strains.
Additionally, the zebrafish model offers a number of technical advantages including ease and cost of maintenance, rapid development, high fecundity, optical transparency of embryos supporting phenotypic screening, existence of many mutant strains, or amenability for both forward and reverse genetics.
See Supporting Information for a list of mutant strains used for complementation tests to identify causal TS Eos/ Ste mutations (Table S2).
This observation was partly expected for aerobic conditions and could be attributed to a major reduction in the respiratory capacity of the mutant strain, which is also supported by the carbon limited chemostat experiments.
All three scaffolds were inhibitors of the bc1 complex as demonstrated by their loss of potency to the seven qcrB mutant strains and the laboratory-adapted H37Rv strain (Table S7 in the Supporting Information ).
These three mutant strains each carried a substitution or nonsense mutation in at least one DNA repair gene (Supporting Information, File S1).
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