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The results clearly show that the mutant strains produced high-efficient cellulases that are stable at a broad pH range at very high temperatures.
However, another group using similar gene knockout methods to generate mutant strains produced conflicting findings with a role for both toxins A and B [ 65].
Similar analysis of the insertional mutant strains produced comparable results even though the growth conditions used in the two experiments were slightly different (Additional file 1: Figure S4).
The Y. lipolytica mutant strains produced in this study warrant further investigation based on successful growth and production of valuable bioproducts on protein, sugars, and coffee waste substrates.
However, both cciI and cciR mutant strains produced more luminescence than the wild type K56-2 strain unlike in the liquid assay (Fig. 1A).
Rodriguez and Smith reasoned that IrtA and B may be involved in mycobactin synthesis; however, the irtA mutant and irtAB double mutant strains produced equivalent amounts of mycobactin compared to the wild-type strain [ 105].
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These mutant strains produce deficiencies in IL-4 and IL-13 functions, respectively.
All the mutant strains produce the sesquiterpene pentalenene and show no difference in growth when compared to the wild-type strain.
For the identification of hybrid perithecia, mutant strains producing black spores were crossed against mutant strains carrying in addition the spore color marker fus1-1.
Since the mutant strains produce fewer transcripts, more resources for host transcription and translation may be available, thus increasing host growth rate.
The abaA mutants produce aberrant phialides that fail to produce conidia, whereas wetA mutant strains produce conidia, but these fail to mature and eventually autolyze (Adams et al. 1988; Clutterbuck 1969; Ogawa et al. 2010; Timberlake et al. 1987).
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