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To investigate the role of each gene of pilus island 2a (Figure 6A) in biofilm formation, we also analyzed biofilm formation in the mutant strains, generated in strain 515, lacking either one of the three structural LPXTG proteins (BP, AP1 and AP2) or the two SrtC-like sortases SrtC-1 and SrtC-2 [22].
Two mutant strains generated from two independent recombinant embryonic stem cell clones were analyzed.
The roles of the various mechanisms were also investigated by simulating the stochastic model on in silico mutant strains generated by eliminating individual mechanisms.
We employed both C. graminicola wild type (WT) strain CgM2 and mutant strains generated by Agrobacterium tumefaciens-mediated transformation (ATMT), which are affected in virulence to different extent.
Intriguingly in the Δ Bar1 mutant strains generated in this study, sporulation resulted in the formation of shorter, less uniform spores with fewer cells than the WT (Rittenour et al., 2011).
We have now used a whole genome resequencing approach with mutant strains generated from two independent genetic screens to determine the full mutagenic spectrum of 4-NQO in A. nidulans.
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We used CAMI to introduce each of the four plasmid mixes into each of the six mutant strains, generating 24 lines, allowing us to look for phenotypes in a total of 78 mutant neuron classes (4 transgenic lines × 6 mutants × 3 neuron classes per line).
In this study, we constructed a Δ cspA A. fumigatus strain by targeted gene disruption mediated by Agrobacterium tumefaciens, along with a complemented mutant strain generated by homologous introduction of an intact cspA sequence.
Recently, the screening of chemical libraries in panels of knockout collections of S. cerevisiae, for unravelling chemical synthetic lethality relationships, has been complemented through usage of collections of mutant yeast strains generated by meiotic recombination (Perlstein et al, 2006).
Mutant strains were generated by transposon mutagenesis.
The mutant strains were generated using standard genetic and molecular biology techniques.
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