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In the supplementary data results from microbial growth morphology, zymographic analysis and enzyme activity profile of cellulose degrading enzymes from the wild-type and mutant strains during growth in different growth carbon substrates is shown.
Having established that there were no differences in growth rates between wild-type and mutant strains during the 480 minute acclimation period from 25°C to 38°C, PSII activity and thermotolerance under conditions of thermal acclimation were examined.
We provide CLS data for both wild-type and mutant strains during long-term quiescence.
We used flow cytometric analysis of fixed and propidium iodide stained cells to examine the morphological heterogeneity of the wild-type (FC36) and isogenic mutant strains during the course of our experiments.
Even though initiation of symbiosis was not affected by the altered NF structure in the mutant, and nodules were formed at an equal rate for both wild type and mutant strains during the first two weeks post inoculation, the final number of nodules was significantly lower for the mutant compared with the wild type at the end of the experiment.
To capture the various levels of expression observed experimentally for the HSE-CYC1-lacZ reporter, the number of functional states for each node was determined by the maximum number of statistically-significant different groups of ß-galactosidase activity displayed experimentally by the whole panel of WT and PKA-RN mutant strains during exponential phase (Figs. 2, 4, 5, and 6).
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It was previously reported that MpkA is important for regulating siderophore production by measuring an increased amount of siderophores in the Δ mpkA mutant strain during iron starvation [ 8].
Examination of PSII activity following thermal challenge at 46°C for 1 hour demonstrates that the residual PSII activity in wild-type and all mutant strains increases during acclimation to 38°C over a 480 minute period (Table 3).
The mutant strains engineered during this study were constructed using the PCR one-step λ Red recombinase method (Datsenko and Wanner, 2000).
Fig. 7 Comparison of cell growth (a) and organic acid production (b) with the mutant strains and the parent strain during preliminary screening Fig. 8 Comparison of l-lactic acid production with mutant strains (L. paracasei S1 to S6) and the parent strain (L.
The results obtained in these inoculations were in agreement with those previously described, indicating that the deficiency suffered by the mutant strain arise during the plant infection (data not shown).
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