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Various Escherichia coli mutant strains designed for succinate production under aerobic conditions were characterized in chemostat.
The metabolic engineering strategies identified in silico resemble existing mutant strains designed for the synthesis of the respective products.
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A three-step gene splicing by overlap extension was used to generate the DR0615 mutant strain (designed MOxyR) [43].
The three mutant strains were designed to synthesize different amounts of Rfc by altering the ribosome-binding sequence and start codon.
To this end, we compared transgene silencing in wildtype animals with that occurring in mutant strains lacking all ADAR activity, as well as to that occurring in strains designed to express even higher concentrations of dsRNA.
The 'large-scale survey' experiment type is used for high-throughput screens involving large collections of mutant strains whose design and construction depended on knowledge of the genome sequence.
We integrated three deletion mutants of TFG2 into a yeast strain designed for phenotyping rpo21 / rpb1 alleles.
In order to uncover the regulome of HrpX transcription regulator by profiling the wild-type and the hrpX mutant strains transcriptome, we had designed a microarray chip covering the whole genome under Agilent platform in our previous study [ 33].
Systems metabolic engineering guided by flux balance analysis (FBA) was first employed to rationally design mutant strains of Scheffersomyces stipitis with high biomass yield.
To simulate and understand these properties are especially important for metabolic engineers to design mutant strains that remove these regulatory mechanisms that protect microorganism from over-production to increase yield of amino acid production.
The forward primer was designed from the conserved region common to both wild-type and mutant strains; the reverse primers were designed specifically for wild-type and mutant strains, respectively, derived from the 3´ terminal base of each primer.
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