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Comparison of this strain with panD2 mutant strains derived from E. coli SJ16 showed an equivalent dependence on exogenous β-alanine for growth in liquid medium.
We have been able to identify all of the elements that cause contig breaks flanking unique sequence in mutant strains derived from strain NCM3722.
Our recent studies have shown that light influences the mycelial growth of Hypocrea but the effect varies depending on the species: temperate H. atroviridis is strongly stimulated by illumination [33] while mutant strains derived from tropical T. reesei are frequently photoinhibited [34].
To assess the role of superoxide dismutases, the isogenic series of the sod1Δ, sod2Δ and sod1Δ sod2Δ mutant strains derived from the S. cerevisiae EG103 wild type strain were used in zone inhibition assay with CTBT (5 μg per disk) on YPD.
Constant-mass, carbon-limited, chemostat-type continuous fermentations were used to cultivate the parent strain T. reesei QM 9414, and the Δlae1 and OElae1 mutant strains derived from it on glucose (1%, w/v) as a sole carbon source at two different dilution rates (D = 0.075 h-1 and D = 0.020 h-1).
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JPU is a ura3 mutant strain derived from S. cerevisiae JP1, an industrial strain isolated from Japungu Agroindustrial distillery (Santa Rita-PB, Rita-PB, Brazilosited andthe depositedt of Mycology Culture Collection (Universidate Federal de Pernambuco, Brazil).
As strain KF-25 is a wild isolate and Wisconsin 54-1255 is a mutant strain derived from NRRL 1951 [ 13], the different origins might cause the different physiological features.
Proline-using mutant phenotypes in strains derived from RT244 were mapped by meiotic crossing to RT250 (yA1 pabaA1 pyrgpdAgpdareAHAreAHA fmdS-lacZ prn-309).
All deletion mutant strains were derived from TB123.
Colonization with SC5314 was equivalent to that of CAI4, the parental strain from which the mutant strains were derived (Supporting Information Fig. S1B).
We suspect that the nanoparticles present in the mutant strains are derived from vacuolar FeIII in which the pH of the organelle is higher than normal.
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