Sentence examples for mutant strains created from inspiring English sources

All mutant strains created in the course of this study are still able to mate with CBS999.97 and also with QF1 despite their deletions.

Plasmid pJC1 was transformed into the parent strain, creating the att:: hdrABC* strain, as well as into the Δ hdrABC deletion mutant strain, creating the Δ hdrABC att:: hdrABC* mutant strain (see Fig. S1 in the supplemental material).

C65S, C65A, and C65P mutant strains were created.

S. oneidensis mutant strains were created as described previously [28].

The Nse3 mutant strains were created using Cre recombinase-mediated cassette exchange, as detailed in Watson et al. [32].

ΔppsA and ΔpckA mutant strains were created by deletion mutagenesis of wild-type S. Typhimurium using a chloramphenicol cassette as described [76].

The E. coli murA (RM345) and ftsZ (RM349) deletion mutant strains were created using the Lambda Red recombinase system with a derivate of pKD4 as a template (Datsenko and Wanner, 2000).

Alternatively, considering that the chemical mutagens with which most of these mutant strains were created cause specific nucleobase alterations (e.g., G→A by EMS), certain septin proteins could be predisposed to acquire Ts– mutations if the codons representing critical residues are easily mutated to nonconservative amino acid substitutions (e.g., GGA→AGA and Gly→Arg).

An aox1 mutant strain was created using targeted gene disruption, and the mutant strain was reconstituted to wild type using a full-length AOX1.

The ΔlaeAΔrsrA double mutant strain was created by crossing TJW131.1 with RJW135.1 to obtain RJW273 and confirmed by PCR.

Since S. oralis is readily transformable, a ciaR mutant strain was created by integrating a resistance marker into the gene.