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Both single and double mutant strains also promoted growth of cucumber plants in the absence of added N-fertilizer, while this property was only marginal in the parental strain.
Both pmt4 mutant strains also demonstrated striking growth inhibition by the addition of 0.7 M or 1 M NaCl to the medium (Figure 6A).
The decreased protein level in the mutant strains also suggests that the proteins are getting rapidly degraded.
The mutant strains also were grown on media without Congo red to ensure that this effect was dependent on the presence of dye (data not shown).
Both single mutant strains also showed an increased susceptibility to bacitracin, but MIC values 15-200 μg/ml for H1003 (ΔSCO3089-3090) and 30-35 μg/ml for H1002 (ΔSCO3110-3111)) were not as low as for the double mutant.
As the plots show, the great majority of the cells in all the cultures were approximately the same size as the wild-type cells, but the cultures of the mutant strains also contained larger, presumably filamenting cells.
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The ackA-pta mutant strain also exhibits an increased lactate synthesis rate.
The mutant strain also failed to agglutinate chicken erythrocytes [5].
In the present study, we confirmed our previous finding that ΔsecA2 conferred enhanced protection against virulent challenge when compared with BCG, and demonstrated that vaccination with the ΔsecA2ΔlysA double mutant strain also gave protection that was significantly better than that achieved with BCG.
Each resistant mutant strain also harbored nonoverlapping mutations, indicating that these strains are not siblings.
Since this enhanced transcription in the mutant strain also occurs in darkness with ubi4 and tre41025, these genes seem to be subject to a general repression by ENVOY.
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