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This result suggests that telomerase activity is lower in the K75A mutant strain, even in the absence of shelterin formation.
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Second, in the mutant strains even at position A, there was no evidence of the highly aligned phenotype (Fig. 2D); thus, they were unable to exhibit the high accuracy projection behavior at steep gradient slopes of wild-type cells.
Defects in growth and development were observed in different and independent mutant strains, even after removal of many background mutations by backcrossing against wild-type animals.
Growth of the mutant strain was even detected at an initial crude glycerol concentration of 105 g/l.
However, knockout of the pfl gene individually could only enhance lactate production while the succinate concentration in the pfl mutant strain was even slightly lower when compared to the control strain [ 52].
Nevertheless, the very low frequency of expansion events observed in a wild-type host (∼1%) makes statistically significant analyses of altered expansion proportions in mutant strains difficult (even when analyzing a total of 960 individual colonies per strain).
Compared with liquid media, the mutant strain showed an even stronger filamentation defect on/in the agar media.
Improved astaxanthin productivity of the mutant strain was maintained even when grown on a large scale and holds promise as the basis for viable commercial production of this valuable biochemical by natural means.
In principle, the modified virus that Feldmann developed could incorporate proteins from a mutant strain of Ebola, or even a novel species.
Furthermore, the droxyR could not complement the defect in the E. coli oxyR mutant strain (GS09) (Figure S1), even when GS09 was complemented with droxyR::T201C, which is put the missing cysteine back in DrOxyR (data not shown).
The observed instability of the naturally short-lived Δpp65 protein in the multiple protease-deficient mutant strain S86c could not even be prevented in mutant hosts defective in components of the ubiquitin-proteasome-system (UPS) nor in a yeast Δpep4 mutant devoid of vacuolar proteases (data not shown).
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