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Equations (6) make the implicit assumption that the increased viremia of the mutant strain does not significantly increase its sexual transmission rate.
If a reconstructed mutant strain does not have the expected phenotype, this could result from the lack of a specific interacting allele in the host strain, or possibly a missing non-core gene.
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In contrast, the CDT isogenic mutant strain did not induce caspase 3 above background levels (Fig. 1C).
Like folM, a folX deletion did not change the MIC in trimethoprim, but the mutant strain did have a competitive advantage over the wild-type strain in trimethoprim (Figure 2).
Sixteen of the 27 genes whose expression is significantly reduced in the sigT mutant, and the two genes with increased expression in the mutant strain, do not show SigT-dependency under osmotic stress, as reported by Alvarez-Martinez et al. [28].
Moreover, this mutant strain did not induce an altered cytokine profile in DCs (Driessen et al., 2009).
In addition, cultures of the mutant strain did not achieve the same final optical density as the parent strain in the M9 medium with glycerol.
However, the spo0A mutant strain did not appreciably benefit from introduction of this synthetic pathway and the cellulose utilization efficiency did not further increase.
In S. cerevisiae, the atg11Δ atg17Δ double mutant strain did not contain any detectable autophagic bodies and had a severe autophagy defect (Cheong et al., 2008).
The results demonstrate that the ΔtolC mutant and its complement strain displayed similar growth kinetics to the WT strain, indicating that the ΔtolC mutant strain did not present a growth defect.
Western blot analysis of cell extracts from the Rpn12 mutant strain did indeed show an accumulation of ubiquitylated proteins, suggesting impaired proteasomal function presumably as a consequence of the reduced incorporation of Rpn10 in the proteasome [ 23].
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