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The applications of biotechnology varies from the development of hybrid plant and genetically modified disease resistant mutant strain containing seeds for high yielding superior quality plants to the production of vaccines, including DNA vaccine.
Δlsp1 pBBR), Δlsp2 pBBR), and Δlsp3 pBBR) are the respective mutant strain containing the empty vector, pBBR1-MCS5.
Cutinase was excluded as a pathogencity factor for M. grisea on the basis that a mutant strain containing a non-functional cutinase-encoding gene was still able to cause rice blast disease [18].
An additional mutant strain containing a reduced chitinase version containing just one catalytic and one chitin-binding domain showed diminished growth on chitin in comparison to the mutant containing the single large enzyme.
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Complementation assays, in which the mutant strain contained a new copy of the missing gene elsewhere on the chromosome (Supplementary Table 1), restored the system to that of the WT (Supplementary Fig. 2a and b).
The monosaccharide analyses confirm that curdlan from both wild and mutant strain contains only glucose units.
The mutant strain contains two mutated copies of the relB2 gene, and has the relE2 gene placed away from its promoter, also disrupting the translational coupling that appears to exist in this pneumococcal operon [33].
In other words, if a given mutant strain contains 100 mutations relative to the parent, we assume that only one of those mutations is causal (i.e. needs to be in a true target gene).
Notably, the clpP mutant strain contained wild-type levels of Rot suggesting that the reduction in rot transcription observed in clpX and clpP mutant strains is not reflected in the cellular Rot level, and hence that the ClpP independent effect of ClpX in controlling Rot synthesis is achieved mainly at the post-transcriptional level.
The phase-contrast images in Figure 5B show that, like the recG258 mutant strain, the population of the ruvA60 mutant strain contained filamenting cells, although again, most cells were of normal size and shape.
Others have examined mutant strains containing deletions in outermembrane proteins that show biofilm formation in the animal pathogen B. bronchiseptica is protein-mediated [18].
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