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Table 1 Specific activity of the mutant strain after final optimization S. no.

The dry weight measurements performed at 20 hr showed a consistent and significant 6.5% increase for irradiated melanized samples (P = 0.02) while there was no difference in weight for the mutant strain after irradiation.

Eightfold respective sevenfold increases of amoebae were estimated in the presence of wild or mutant strain after 14 days (Fig. 1).

Although saturating transposon mutagenesis experiments predicted the cspC gene to be essential in C. crescentus [ 15], we were able to obtain a Δ cspC::Ω Spec R mutant strain after screening hundreds of clones [ 11].

A comparison of the differentially up or down regulated genes in the parental and NPK mutant strains again demonstrated that the transcriptome of the parental strain was more distinct from the NPK mutant strain after 24 h culture in the presence of AVICEL, while of the two NPKs mutant strain, ∆ snfA demonstrated the least similarity to the parental strain.

The genomes of the mutant strains after outcrossing twice against N2 were sequenced using Illumina sequencing.

However, NTH1 expression increases around 4.0-fold in gcr1 mutant strains after glucose addition to cells grown on lactate and glycerol.

We therefore compared Eco1 levels between wild type and cohesion establishment factor mutant strains, after arrest in early S-phase using HU.

While the peak of vesicle secretion in the WT strain occurred after 18 h, detection of sterols in vesicle fractions from the mutant strain stagnated after 3 h (Figure 6D).

In marked contrast to the wild-type strain, GFP production could be detected in the ∆yeaG mutant strain population after only 2 h into 'recovery' growth and fluorescence levels increased at a constant rate over time.

A mutant strain obtained after γ-irradiation was used for genome shuffling with ribosome engineering mediated by streptomycin resistance.