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Therefore, for further clinical application, this assay may be useful for drug discovering in IN drug resistance stains by cloning of mutant stains derived from patients and screening for effective therapeutic IN inhibitors.
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Theoretically, the heterozygous mutant line derived from an ALS patient is expected to have 50% WT and 50% mutant FUS.
Other approaches include vaccines made from neoantigens — mutant proteins derived from the patient's tumour.
The ygl138 mutant was derived from Nipponbare treated by ethyl methanesulfonate (EMS).
Cell lines were then co-cultured with isogenic mutants derived from multiple parental stains of H pylori, null for candidate cagPAI genes and vacA.
The Epistatic circler (Ecl) is a complex mutant derived from wildtype SWR/J and C57L/J mice.
The UV-induced mutant PA6609 was derived from PAO6049 (Hohnadel et al., 1986); PAO6049 is a Tn5-induced methionine auxotroph derived from PAO1 (Rella et al., 1985).
Phage mutant 812K1/420 was derived from phage 812K1 by selection on human MRSA strain NRL Atb 420 (St179t179).
NS1-N81 mutant virus was derived from PR8 WT and expresses only the first 81 amino acids of NS145.
Phage mutant 812a was derived from the wild-type phage 812 by selection on S. aureus strain NCTC 8511 (St118, t118) laboratory lysogenized by phage ϕ5347.
Fig. 2 Wild F. fujikuroi NCIM 1019 strain and morphological mutant Mut-4 derived from it.
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