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DesHDAP1, which shows significant similarities to BF2 in terms of secondary structure, translocates effectively across lipid vesicle and bacterial membranes, while the DesHDAP1 proline mutant shows reduced translocation abilities and antimicrobial potency.
Notably the bopA mutant shows reduced virulence in mouse melioidosis models, suggesting that its role in intracellular survival is critical for the pathogensis of B. pseudomallei.
Furthermore, AtHscB is highly expressed in anthers and trichomes and an AtHscB T-DNA insertion mutant shows reduced seed set, a waxless phenotype and inappropriate trichome development as well as dramatically reduced activities of the iron-sulfur enzymes aconitase and succinate dehydrogenase.
The mms21-1 mutant shows reduced pollen number, and viability, and germination and abnormal pollen tube growth.
In Drosophila, a deco mutant shows reduced staining for the cohesin subunit Scc1 at prometaphase of mitosis [ 21].
Knockout of all three sodB genes in A. tumefaciens results in avirulence, while only the sodBI mutant shows reduced virulence when targeted individually [ 61].
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In low iron medium, the Rv3371 deletion mutant showed reduced formation of TAG containing extracellular vesicles.
This T-DNA-mediated OsLCD knockout mutant showed reduced grain Cd accumulation by insertion into the first intron of OsLCD, having no negative effects on grain yield (Shimo et al. 2011).
However, the same mutant showed reduced killing of C. elegans [35].
The Δppk2 mutant showed reduced VBNC formation under formic acid stress compared to WT (Figure 4).
All mutants could be readily isolated from the CAM and no mutant showed reduced fungal burden within the CAM.
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