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As an example of Case 2, in the mutant shown in Code 4 (Additional file 1: Figure S4), w 2 is associated with both original (Code 3 - Additional file 1: Figure S3) and mutated files.
As an example of Case 1, in the mutant shown in Code 2 (Additional file 1: Figure S2), w 1 was reported in the mutated line 4, and w 1 was not reported in correspondent line 4 of the original file (Code 1 - Additional file 1: Figure S1).
Interestingly, as opposed to the dramatic attenuation of the Y. pestis nlpD mutant shown in the present study, the Y. enterocolitica nlpD mutant displayed only a limited impairment of virulence [45].
Therefore, it is likely that the deletion of HST4 would enhance the sectoring phenotype of the hst3Δ mutant shown in Figure 7A.
This seems inconsistent with the lack of endogenous BiP interaction with the ADDA mutant shown in lane 5 and 6 in Figure 5C.
In both cell lines the location of D316K and K138A was quite distinct from the active enzyme, appearing to be entirely cytosolic (exemplified by the D316K mutant, shown in Figs. 1F and 2D and H).
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c, Discharge activity towards Tris and glycerol for all of the tested MYCBP2(cat) cysteine-to-serine mutants (selected mutants shown in Fig. 2c).
We have generated the three carboxyl-terminus deletion mutants shown in panel D. The wild type and mutant pCDNA3.1-Flag-ARIP3 constructs were co-transfected with pRK7-HA-Tollip cDNA.
The sporulation phenotype of rdrp-1Δ and rdrp-2Δ mutants shown in Figure 1A reinforces that involvement.
I constructed various L1 mutants shown in Figure 1A and determined the ubiquitination levels for each mutant by comparing the mutant with wild-type L1 (L1WT, Fig. 1B).
All localization mutants (shown in blue) are different from the σF mutant control with p values less than 0.0025 from a paired t-test.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com